8 Plasmid Cloning Procedures You Need to Know
The basic procedure performed by any molecular biologist is, naturally, cloning. You may wish to clone a DNA fragment into a plasmid for many purposes, such as protein expression and purification, gene knock out / silencing / overexpression, reporter gene fusion to a gene of interest, and many more. Whether you plan on doing one thing or another with the plasmid you cloned, when it comes to the cloning procedure you will need to perform the same steps, which are the ABC’s of molecular biology.

If there is something that students quarrel passionately about it is over shared resources. Now, “resource” is a BIG term covering everything from tips for your pipettes to your PI’s time. Today I will talk about shared equipment and how to try and manage the lab in such a way that your machines will serve you efficiently.
Happy New Year, everyone! All of us at Labguru hope you had a wonderful holiday season, and are ready for successful and prosperous research experiments in 2012. With every new year, of course, comes New Year’s resolutions—things we hope to do differently than we did in the past or perhaps some new positive additions to our routines. Nowhere is this more applicable for researchers than the place they spend the majority of their time: The lab.